Genome-wide Gene Deletions in <em>Streptococcus sanguinis</em> by High Throughput PCR
نویسندگان
چکیده
منابع مشابه
Genome-wide gene deletions in Streptococcus sanguinis by high throughput PCR.
Transposon mutagenesis and single-gene deletion are two methods applied in genome-wide gene knockout in bacteria (1,2). Although transposon mutagenesis is less time consuming, less costly, and does not require completed genome information, there are two weaknesses in this method: (1) the possibility of a disparate mutants in the mixed mutant library that counter-selects mutants with decreased c...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2012
ISSN: 1940-087X
DOI: 10.3791/4356